Hotspot

orf337

Genome · 106-1119 · 1014 bp

Back to Klebsormidium

Pi 0.464

MarkerSeek score 83.40

Primer available yes

Label orf337

Feature Metadata

label_name: orf337
feature_type: gene
region: Genome
length_bp: 1014
variable_sites: 443
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf337_p1	GGCGTAAACCCCGTCATAAT	TCACCATTTGCCTCTTGCTT	50.0	45.0	58.0	58.0	1706 bp	0.5	59.7
orf337_p2	GGCGTAAACCCCGTCATAAT	TAGCGGGTATGGTCTAGTGG	50.0	55.0	58.0	58.0	1562 bp	0.5	59.6
orf337_p3	GGCGTAAACCCCGTCATAAT	TTGGGTCGTTTGTTTTTGGC	50.0	45.0	58.0	58.0	1407 bp	0.5	59.6
orf337_p4	GGCGTAAACCCCGTCATAAT	CAAGAGCTCCCCATCATTCC	50.0	55.0	58.0	58.0	1244 bp	0.5	59.5
orf337_p5	GGCGTAAACCCCGTCATAAT	AGCGGGGTACTAAAAGACCT	50.0	50.0	58.0	58.1	1359 bp	0.5	59.2