Hotspot

psaI-odpB

Genome · 194944-195580 · 637 bp

Back to Klebsormidium

Pi 0.275

MarkerSeek score 83.70

Primer available yes

Label psaI-odpB

Feature Metadata

label_name: psaI-odpB
feature_type: igs
region: Genome
length_bp: 637
variable_sites: 166
pi: 0.275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-odpB_p1	GTTGTCCCATACGAACCCTC	TACCTGGCTTGCAATTGGTT	55.0	45.0	58.0	58.0	1383 bp	0.5	59.8
psaI-odpB_p2	CCCTCGACTCTCTATCTGCA	TACCTGGCTTGCAATTGGTT	55.0	45.0	58.0	58.0	1778 bp	0.5	59.7
psaI-odpB_p3	GTTGTCCCATACGAACCCTC	ACTCCTTACAGTGGTCTGCT	55.0	50.0	58.0	58.0	910 bp	0.5	59.7
psaI-odpB_p4	CCCTCGACTCTCTATCTGCA	ACTCCTTACAGTGGTCTGCT	55.0	50.0	58.0	58.0	1305 bp	0.5	59.7
psaI-odpB_p5	TTTCGCTCTTGACCCGAAAA	TACCTGGCTTGCAATTGGTT	45.0	45.0	58.0	58.0	1433 bp	0.5	59.6