Hotspot

trnF(gaa)-ycf4

Genome · 192643-194200 · 1558 bp

Back to Klebsormidium

Pi 0.42

MarkerSeek score 71.50

Primer available yes

Label trnF(gaa)-ycf4

Feature Metadata

label_name: trnF(gaa)-ycf4
feature_type: igs
region: Genome
length_bp: 1558
variable_sites: 276
pi: 0.42
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-ycf4_p1	GGTATTAGCAGCCGTTTCCA	TGCAGATAGAGAGTCGAGGG	50.0	55.0	58.0	58.0	2441 bp	0.5	59.5
trnF(gaa)-ycf4_p2	ACCGTACAACTTGCATGTGT	TGCAGATAGAGAGTCGAGGG	45.0	55.0	58.0	58.0	2341 bp	0.5	59.5
trnF(gaa)-ycf4_p3	TTCAGTCCCATGCTCTACCA	TGCAGATAGAGAGTCGAGGG	50.0	55.0	58.0	58.0	1776 bp	0.5	59.4
trnF(gaa)-ycf4_p4	GGTATTAGCAGCCGTTTCCA	TTTTCGGGTCAAGAGCGAAA	50.0	45.0	58.0	58.0	2786 bp	0.5	59.4
trnF(gaa)-ycf4_p5	ACACACAAAGCGCTAAGTCA	TGCAGATAGAGAGTCGAGGG	45.0	55.0	58.1	58.0	2209 bp	0.5	59.4