Hotspot

psbJ-orf342

Genome · 79238-79924 · 687 bp

Back to Klebsormidium

Pi 0.427

MarkerSeek score 83.40

Primer available yes

Label psbJ-orf342

Feature Metadata

label_name: psbJ-orf342
feature_type: igs
region: Genome
length_bp: 687
variable_sites: 276
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-orf342_p1	TACCCTCGCTTTTTGTTGCT	GATGTGGCCGAAGCTAGAAA	45.0	50.0	58.0	58.0	1442 bp	0.5	59.6
psbJ-orf342_p2	TACCCTCGCTTTTTGTTGCT	ATGTGGCCGAAGCTAGAAAG	45.0	50.0	58.0	58.0	1441 bp	0.5	59.6
psbJ-orf342_p3	TACCCTCGCTTTTTGTTGCT	TAATGTGGATGTGGCCGAAG	45.0	50.0	58.0	58.0	1449 bp	0.5	59.5
psbJ-orf342_p4	TACCCTCGCTTTTTGTTGCT	TTCTGACTGGAAAACCGGTG	45.0	50.0	58.0	58.0	1527 bp	0.5	59.5
psbJ-orf342_p5	TACCCTCGCTTTTTGTTGCT	TCGACAAGTTCTAACGCGAG	45.0	50.0	58.0	58.0	2062 bp	0.5	59.5