Hotspot

trnS(uga)-psbZ

Genome · 50333-50923 · 591 bp

Back to Klebsormidium

Pi 0.267

MarkerSeek score 83.40

Primer available yes

Label trnS(uga)-psbZ

Feature Metadata

label_name: trnS(uga)-psbZ
feature_type: igs
region: Genome
length_bp: 591
variable_sites: 144
pi: 0.267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(uga)-psbZ_p1	GAGCCAATTTCGGAAGCCTA	AAGTAAGCCAAACCACAGGG	50.0	50.0	58.0	58.0	1698 bp	0.5	59.7
trnS(uga)-psbZ_p2	GAGCCAATTTCGGAAGCCTA	GAGAAGCCAAGATCACTGGG	50.0	55.0	58.0	58.0	1194 bp	0.5	59.4
trnS(uga)-psbZ_p3	GAGCCAATTTCGGAAGCCTA	GGAGAAGCCAAGATCACTGG	50.0	55.0	58.0	58.0	1195 bp	0.5	59.4
trnS(uga)-psbZ_p4	TCAAGACCGGAACAATCGTC	AAGTAAGCCAAACCACAGGG	50.0	50.0	57.9	58.0	1220 bp	0.5	59.4
trnS(uga)-psbZ_p5	GAGCCAATTTCGGAAGCCTA	AGCAAGTAAGCCAAACCACA	50.0	45.0	58.0	57.9	1701 bp	0.5	59.2