Hotspot

trnQ(uug)-rps12

Genome · 35317-35857 · 541 bp

Back to Klebsormidium

Pi 0.307
MarkerSeek score 84.50
Primer available yes
Label trnQ(uug)-rps12

Feature Metadata

label_name
trnQ(uug)-rps12
feature_type
igs
region
Genome
length_bp
541
variable_sites
158
pi
0.307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ(uug)-rps12_p1 GCTATTCGGAGGTTCGAGTC TGGCTTTTTAGGCGTAGTTGT 55.0 42.9 57.9 58.1 582-594 bp 1.0 81.5
trnQ(uug)-rps12_p2 TATTCGGAGGTTCGAGTCCT TGGCTTTTTAGGCGTAGTTGT 50.0 42.9 57.6 58.1 580-592 bp 1.0 79.1
trnQ(uug)-rps12_p3 ATTCGGAGGTTCGAGTCCTT TGGCTTTTTAGGCGTAGTTGT 50.0 42.9 58.4 58.1 579-591 bp 1.0 79.0
trnQ(uug)-rps12_p4 GCTATTCGGAGGTTCGAGTC TGGCTTTTTAGGCGTAGTTG 55.0 45.0 57.9 56.4 582-594 bp 1.0 77.5
trnQ(uug)-rps12_p5 GCTATTCGGAGGTTCGAGTC GGCTTTTTAGGCGTAGTTGT 55.0 45.0 57.9 56.3 581-593 bp 1.0 77.5