Hotspot

orf337-trnG(gcc)

Genome · 1120-4499 · 3380 bp

Back to Klebsormidium

Pi 0.486

MarkerSeek score 79.60

Primer available yes

Label orf337-trnG(gcc)

Feature Metadata

label_name: orf337-trnG(gcc)
feature_type: igs
region: Genome
length_bp: 3380
variable_sites: 1537
pi: 0.486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf337-trnG(gcc)_p1	ACACGTCGTTTTCAAGGTGA	CGTCGACTTGGATACGGATC	45.0	55.0	58.0	58.0	1012 bp	0.5	59.6
orf337-trnG(gcc)_p2	TCACACGTCGTTTTCAAGGT	CGTCGACTTGGATACGGATC	45.0	55.0	58.0	58.0	1014 bp	0.5	59.6
orf337-trnG(gcc)_p3	ACACGTCGTTTTCAAGGTGA	ATACGGATCTTGGTGAACGC	45.0	50.0	58.0	58.1	1001 bp	0.5	59.4
orf337-trnG(gcc)_p4	TCACACGTCGTTTTCAAGGT	ATACGGATCTTGGTGAACGC	45.0	50.0	58.0	58.1	1003 bp	0.5	59.4
orf337-trnG(gcc)_p5	ACACGTCGTTTTCAAGGTGA	AGCGTTCCGACTCATTGATC	45.0	50.0	58.0	58.1	1148 bp	0.5	59.3