Hotspot

rpoC2

LSC · 18780-22913 · 4134 bp

Pi 0

MarkerSeek score 24.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4134
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GCGGAATTTCTACGCGAATC	TGCAGTACCCCCTGTGAATA	50.0	50.0	57.7	58.0	1146 bp	1.0	77.4
rpoC2_p2	GCGGAATTTCTACGCGAATC	GTACGTGTCGGATGAACCAA	50.0	50.0	57.7	57.9	1214 bp	1.0	77.2
rpoC2_p3	GCGGAATTTCTACGCGAATC	ACGTGTCGGATGAACCAAAT	50.0	45.0	57.7	57.8	1212 bp	1.0	76.3
rpoC2_p4	GCGGAATTTCTACGCGAATC	TACCCCCTGTGAATACTCCG	50.0	55.0	57.7	58.2	1141 bp	1.0	76.0
rpoC2_p5	GCGGAATTTCTACGCGAATC	GTGAATACTCCGCCGGTATG	50.0	55.0	57.7	58.2	1133 bp	1.0	76.0