Hotspot

rbcL

LSC · 54933-56366 · 1434 bp

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Pi 0
MarkerSeek score 60.40
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1434
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCATGAGTTGTAGGGAGGGA TATGCATGTTGGATTCGGCT 50.0 45.0 57.1 57.7 1537-1562 bp 1.0 71.0
rbcL_p2 ACGCCTAATTCATGTCGAGT TATGCATGTTGGATTCGGCT 45.0 45.0 57.0 57.7 1585-1610 bp 1.0 70.8
rbcL_p3 TTCATGAGTTGTAGGGAGGGA TATGCATGTTGGATTCGGCT 47.6 45.0 57.8 57.7 1538-1563 bp 1.0 68.9
rbcL_p4 AACGCCTAATTCATGTCGAGT TATGCATGTTGGATTCGGCT 42.9 45.0 57.8 57.7 1586-1611 bp 1.0 68.5
rbcL_p5 TCATGAGTTGTAGGGAGGGAT TATGCATGTTGGATTCGGCT 47.6 45.0 57.6 57.7 1537-1562 bp 1.0 67.1