Hotspot

psbC

LSC · 33098-34519 · 1422 bp

Pi 0

MarkerSeek score 30.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTTTCTCAGGAAATTCGCGC	GGGAACCGAAGTGACTCCTA	50.0	55.0	58.1	58.4	1577 bp	1.0	75.5
psbC_p2	CGTTTCTCAGGAAATTCGCG	GGGAACCGAAGTGACTCCTA	50.0	55.0	57.9	58.4	1578 bp	1.0	75.3
psbC_p3	GTTTCTCAGGAAATTCGCGC	GGAACCGAAGTGACTCCTAC	50.0	55.0	58.1	57.3	1576 bp	1.0	73.9
psbC_p4	CGGCGGAAGATCCTGAATTT	GGGAACCGAAGTGACTCCTA	50.0	55.0	58.3	58.4	1558 bp	1.0	73.8
psbC_p5	AGGTATTCGTGCTTGGATGG	GGGAACCGAAGTGACTCCTA	50.0	55.0	57.7	58.4	1503 bp	1.0	73.8