Hotspot

ndhK

LSC · 48905-49582 · 678 bp

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Pi 0.00147
MarkerSeek score 59.60
Primer available yes
Label ndhK

Feature Metadata

label_name
ndhK
feature_type
gene
region
LSC
length_bp
678
variable_sites
1
pi
0.00147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK_p1 TCTGAAGATTGAGCCACGAC GTTTATGCATGGCGAAAGGG 50.0 50.0 57.6 57.8 814 bp 1.0 75.5
ndhK_p2 CTGAAGATTGAGCCACGACT GTTTATGCATGGCGAAAGGG 50.0 50.0 57.6 57.8 813 bp 1.0 75.4
ndhK_p3 TCTGAAGATTGAGCCACGAC GGGGCATTGGAATGGTCTTA 50.0 50.0 57.6 57.5 796 bp 1.0 73.5
ndhK_p4 CTGAAGATTGAGCCACGACT GGGGCATTGGAATGGTCTTA 50.0 50.0 57.6 57.5 795 bp 1.0 73.5
ndhK_p5 TGAAGATTGAGCCACGACTT GTTTATGCATGGCGAAAGGG 45.0 50.0 57.2 57.8 812 bp 1.0 71.7