Hotspot

ndhF

SSC · 125532-127763 · 2232 bp

Pi 0.00179

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 4
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGTAAGATCCGGTGAATCG	TGTTTCGATGCAACAGCAAG	52.4	45.0	58.0	57.6	2382 bp	1.0	68.8
ndhF_p2	AGGTAAGATCCGGTGAATCG	TGTTTCGATGCAACAGCAAG	50.0	45.0	56.8	57.6	2381 bp	1.0	67.5
ndhF_p3	CGAAAGAGGTAAGATCCGGTG	TGTTTCGATGCAACAGCAAG	52.4	45.0	58.2	57.6	2387 bp	1.0	67.4
ndhF_p4	CGAAAGAGGTAAGATCCGGT	TGTTTCGATGCAACAGCAAG	50.0	45.0	56.7	57.6	2387 bp	1.0	66.9
ndhF_p5	TCGAAAGAGGTAAGATCCGGT	TGTTTCGATGCAACAGCAAG	47.6	45.0	58.3	57.6	2388 bp	1.0	66.8