Hotspot

accD-psaI

LSC · 58388-59060 · 673 bp

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Pi 0.00151
MarkerSeek score 61.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
673
variable_sites
1
pi
0.00151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCCAGAAGGGCTTATTCGATC AAAGAAGCCATTGCGATTGC 47.6 45.0 57.2 58.0 843-854 bp 1.0 67.2
accD-psaI_p2 CCAGAAGGGCTTATTCGATCT AAAGAAGCCATTGCGATTGC 47.6 45.0 57.2 58.0 842-853 bp 1.0 67.1
accD-psaI_p3 TTCCAGAAGGGCTTATTCGA AAAGAAGCCATTGCGATTGC 45.0 45.0 56.2 58.0 844-855 bp 1.0 67.1
accD-psaI_p4 TCCAGAAGGGCTTATTCGATC CCGGAAAGACTAGGCCTACT 47.6 55.0 57.2 58.2 824-835 bp 1.0 65.5
accD-psaI_p5 CCAGAAGGGCTTATTCGATCT CCGGAAAGACTAGGCCTACT 47.6 55.0 57.2 58.2 823-834 bp 1.0 65.4