Hotspot

psbC-trnS-UGA

LSC · 34520-34759 · 240 bp

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Pi 0.0127
MarkerSeek score 61.40
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
240
variable_sites
3
pi
0.0127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 431-435 bp 1.0 80.3
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 348-352 bp 1.0 80.1
psbC-trnS-UGA_p3 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 439-443 bp 1.0 79.9
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 356-360 bp 1.0 79.7
psbC-trnS-UGA_p5 TCTTCGTAGGGCATTTGTGG TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.9 58.1 438-442 bp 1.0 79.2