Hotspot

psbM-trnD-GUC

LSC · 10649-11294 · 646 bp

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Pi 0.0031
MarkerSeek score 60.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
646
variable_sites
2
pi
0.0031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TCTGATTCCCCTGGGATTGT 39.1 50.0 57.6 58.0 785-786 bp 1.0 52.6
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TCTGATTCCCCTGGGATTGT 40.0 50.0 54.5 58.0 779-780 bp 1.0 52.1
psbM-trnD-GUC_p3 ACTAAAATGAACAGTGCAGTAGC TCTGATTCCCCTGGGATTGT 39.1 50.0 57.4 58.0 782-783 bp 1.0 51.2
psbM-trnD-GUC_p4 GGAACTAAAATGAACAGTGCAGT TTCAATAGGCAAGAGCACCG 39.1 50.0 57.6 58.3 763-764 bp 1.0 50.6
psbM-trnD-GUC_p5 AGGAACTAAAATGAACAGTGCAG TCTGATTCCCCTGGGATTGT 39.1 50.0 57.3 58.0 786-787 bp 1.0 50.5