Hotspot

ycf2

LSC · 87080-93343 · 6264 bp

Back to Kitagawia

Pi 0.00875

MarkerSeek score 44.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6264
variable_sites: 105
pi: 0.00875
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATTTCTGAGCAGAAGAGCCG	TTCATCACCACCACCTTGTG	50.0	50.0	58.0	58.0	825-846 bp	1.0	82.2
ycf2_p2	TCGAACCGAGAGATCCATGA	TTCATCACCACCACCTTGTG	50.0	50.0	57.9	58.0	919-940 bp	1.0	81.8
ycf2_p3	TCCATGAATCGGGATCCTGA	TTCATCACCACCACCTTGTG	50.0	50.0	57.9	58.0	906-927 bp	1.0	81.5
ycf2_p4	ATTTCTGAGCAGAAGAGCCG	GATACCCGTGACTCGATTGG	50.0	55.0	58.0	57.9	779-800 bp	1.0	81.1
ycf2_p5	ATTTCTGAGCAGAAGAGCCG	TTGTGCCCTGTCGAAGTAAG	50.0	50.0	58.0	57.8	1229-1250 bp	1.0	80.6