Hotspot

ycf1

SSC · 125898-131384 · 5487 bp

Back to Kitagawia

Pi 0.00789

MarkerSeek score 45.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 85
pi: 0.00789
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGTGGCACAAATTCTGGGA	AGATATTGAGGCCTGGACCA	45.0	50.0	57.6	57.8	229-250 bp	1.0	83.6
ycf1_p2	AGTGGCACAAATTCTGGGAA	AGATATTGAGGCCTGGACCA	45.0	50.0	57.6	57.8	228-249 bp	1.0	83.6
ycf1_p3	AAGTGGCACAAATTCTGGGA	TTGAGGCCTGGACCAAAATG	45.0	50.0	57.6	58.4	224-245 bp	1.0	82.3
ycf1_p4	AGTGGCACAAATTCTGGGAA	TTGAGGCCTGGACCAAAATG	45.0	50.0	57.6	58.4	223-244 bp	1.0	82.3
ycf1_p5	TGGCACAAATTCTGGGAAGA	AGATATTGAGGCCTGGACCA	45.0	50.0	57.3	57.8	226-247 bp	1.0	81.3