Hotspot

psbA

LSC · 294-1355 · 1062 bp

Back to Kitagawia

Pi 0.00402

MarkerSeek score 27.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 8
pi: 0.00402
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	39.1	45.0	56.6	58.0	1137 bp	1.0	45.5
psbA_p2	ACTCGTATATACTAAAACCCGGT	TGCTTGGGAGTCCCTGATAA	39.1	50.0	56.6	58.0	1128 bp	1.0	45.4
psbA_p3	AACTCGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	37.5	45.0	57.3	58.0	1138 bp	1.0	42.8
psbA_p4	ACTCGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	41.7	45.0	57.7	58.0	1137 bp	0.833333	39.2
psbA_p5	ACTCGTATATACTAAAACCCGGTC	TGCTTGGGAGTCCCTGATAA	41.7	50.0	57.7	58.0	1128 bp	0.833333	39.1