Hotspot

rpl32-trnL-UAG

SSC · 115309-116276 · 968 bp

Back to Kitagawia

Pi 0.0249
MarkerSeek score 65.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
968
variable_sites
45
pi
0.0249
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1047-1092 bp 1.0 86.0
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1048-1093 bp 1.0 85.9
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 1068-1113 bp 1.0 85.3
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 1070-1115 bp 1.0 85.3
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1057-1102 bp 1.0 85.0