Hotspot

ndhF-rpl32

SSC · 114111-115146 · 1036 bp

Back to Kitagawia

Pi 0.0174

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1036
variable_sites: 34
pi: 0.0174
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1707-1788 bp	1.0	86.1
ndhF-rpl32_p2	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2037-2055 bp	0.666667	70.0
ndhF-rpl32_p3	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2036-2054 bp	0.666667	70.0
ndhF-rpl32_p4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2265-2283 bp	0.666667	69.5
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2264-2282 bp	0.666667	69.5