Hotspot

ndhC-trnV-UAC

LSC · 51653-52766 · 1114 bp

Back to Kitagawia

Pi 0.0116

MarkerSeek score 57.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1114
variable_sites: 24
pi: 0.0116
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1230-1266 bp	1.0	82.7
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1217-1253 bp	1.0	78.8
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1215-1251 bp	1.0	78.1
ndhC-trnV-UAC_p4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.3	58.1	1225-1261 bp	1.0	77.9
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1212-1248 bp	1.0	77.1