Hotspot

psaA-ycf3

LSC · 42767-43512 · 746 bp

Back to Kitagawia

Pi 0.00891

MarkerSeek score 61.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 12
pi: 0.00891
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	818-866 bp	1.0	83.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	805-853 bp	1.0	82.9
psaA-ycf3_p3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.2	58.1	841-889 bp	1.0	82.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	809-857 bp	1.0	82.7
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	828-876 bp	1.0	82.4