Hotspot

trnT-GGU-psbD

LSC · 32354-33781 · 1428 bp

Back to Kitagawia

Pi 0.00836

MarkerSeek score 69.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1428
variable_sites: 24
pi: 0.00836
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1611-1628 bp	0.666667	67.2
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1607-1624 bp	0.666667	67.0
trnT-GGU-psbD_p3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1618-1635 bp	0.666667	66.4
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	50.0	55.0	57.9	57.8	1614-1631 bp	0.666667	66.2
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	50.0	50.0	58.1	58.2	1619-1636 bp	0.666667	66.0