Hotspot

psbM-trnD-GUC

LSC · 30399-31087 · 689 bp

Back to Kitagawia

Pi 0.0133
MarkerSeek score 61.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
689
variable_sites
18
pi
0.0133
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 50.0 50.0 57.6 58.5 771-800 bp 1.0 77.8
psbM-trnD-GUC_p2 AGGATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 50.0 50.0 57.6 57.0 772-801 bp 1.0 73.6
psbM-trnD-GUC_p3 AGGATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 50.0 52.6 57.6 57.8 770-799 bp 1.0 71.8
psbM-trnD-GUC_p4 GGAACTAGGATGAACAGTGCA TTCAATTGGTCAGAGCACCG 47.6 50.0 57.7 58.5 777-806 bp 1.0 70.2
psbM-trnD-GUC_p5 AGGATGAACAGTGCAGTAGC AGTTCAATTGGTCAGAGCACC 50.0 47.6 57.6 58.5 773-802 bp 1.0 69.7