Hotspot

rpoB-trnC-GCA

LSC · 27007-28221 · 1215 bp

Back to Kitagawia

Pi 0.0139
MarkerSeek score 51.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1215
variable_sites
33
pi
0.0139
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCACGAACCTACAAAAGCCT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.1 58.1 1356-1372 bp 1.0 81.1
rpoB-trnC-GCA_p2 TCCACGAACCTACAAAAGCC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.1 58.1 1357-1373 bp 1.0 81.0
rpoB-trnC-GCA_p3 CCACGAACCTACAAAAGCCT TTATTGATCAGGCGACACCC 50.0 50.0 58.1 57.7 1374-1390 bp 1.0 79.1
rpoB-trnC-GCA_p4 TCCACGAACCTACAAAAGCC TTATTGATCAGGCGACACCC 50.0 50.0 58.1 57.7 1375-1391 bp 1.0 79.0
rpoB-trnC-GCA_p5 CCACGAACCTACAAAAGCCT AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.1 58.4 1338-1354 bp 1.0 78.9