Hotspot

trnS-GCU-trnG-UCC

LSC · 8483-9036 · 554 bp

Back to Kitagawia

Pi 0.0132

MarkerSeek score 56.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 14
pi: 0.0132
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	45.0	55.0	58.0	58.1	640-658 bp	1.0	81.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TAAGGGGGTTACTCGAACGA	45.0	50.0	58.0	57.8	643-661 bp	1.0	80.1
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	45.0	50.0	58.0	58.4	641-659 bp	1.0	78.3
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	50.0	55.0	57.6	58.1	626-644 bp	1.0	78.3
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	55.0	55.0	58.5	58.1	621-639 bp	1.0	77.5