Hotspot

rps16-trnQ-UUG

LSC · 5961-7218 · 1258 bp

Back to Kitagawia

Pi 0.0119
MarkerSeek score 49.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1258
variable_sites
28
pi
0.0119
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT TGGAAGTATAGATGGGGCGT 45.0 50.0 58.0 57.9 1361-1380 bp 1.0 83.2
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT TGGAAGTATAGATGGGGCGT 45.0 50.0 58.0 57.9 1362-1381 bp 1.0 83.2
rps16-trnQ-UUG_p3 TGCTTTCTACCACAGCGTTT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.0 57.9 1875-1894 bp 1.0 82.7
rps16-trnQ-UUG_p4 TTGCTTTCTACCACAGCGTT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.0 57.9 1876-1895 bp 1.0 82.7
rps16-trnQ-UUG_p5 GATTCCCGCGTGATACACTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 2232-2251 bp 1.0 82.0