Hotspot

trnH-GUG-psbA

LSC · 90-293 · 204 bp

Back to Kitagawia

Pi 0.049

MarkerSeek score 81.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 22
pi: 0.049
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	257-273 bp	1.0	89.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	271-287 bp	1.0	88.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	270-286 bp	1.0	88.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	272-288 bp	1.0	88.6
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TGGAAGTTATGCACGAACGT	55.0	45.0	58.1	57.8	292-308 bp	1.0	87.5