Hotspot

rpl14-rpl16

LSC · 82800-82936 · 137 bp

Back to Kirengeshoma

Pi 0.0148

MarkerSeek score 62.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 2
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCATATCGGCGATTGCTAG	TGAAACGGGTGGAGTAACAG	50.0	50.0	58.0	57.5	332-333 bp	1.0	76.7
rpl14-rpl16_p2	CATATCGGCGATTGCTAGCT	TGAAACGGGTGGAGTAACAG	50.0	50.0	58.0	57.5	330-331 bp	1.0	76.7
rpl14-rpl16_p3	AGCATATCGGCGATTGCTAG	ACGGGTGGAGTAACAGAAAC	50.0	50.0	58.0	57.5	328-329 bp	1.0	76.7
rpl14-rpl16_p4	CATATCGGCGATTGCTAGCT	ACGGGTGGAGTAACAGAAAC	50.0	50.0	58.0	57.5	326-327 bp	1.0	76.7
rpl14-rpl16_p5	GAGCATATCGGCGATTGCTA	TGAAACGGGTGGAGTAACAG	50.0	50.0	58.0	57.5	333-334 bp	1.0	76.6