Hotspot

ycf4-cemA

LSC · 61901-62768 · 868 bp

Back to Kirengeshoma

Pi 0.00922

MarkerSeek score 64.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 8
pi: 0.00922
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	980-984 bp	1.0	79.3
ycf4-cemA_p2	GGGGAAGTTGAGCAAAAAGC	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.8	58.1	1007-1011 bp	1.0	78.8
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	994-998 bp	1.0	78.6
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	992-996 bp	1.0	78.3
ycf4-cemA_p5	TTAACTCCCGGGGAAGTTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.3	58.1	1016-1020 bp	1.0	77.9