Hotspot

trnG-GCC-trnM-CAU

LSC · 37407-37592 · 186 bp

Back to Kirengeshoma

Pi 0.0161

MarkerSeek score 61.80

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 3
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	TTGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	329-330 bp	1.0	79.5
trnG-GCC-trnM-CAU_p2	TTGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	293-294 bp	1.0	79.5
trnG-GCC-trnM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	300-301 bp	1.0	79.2
trnG-GCC-trnM-CAU_p4	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	264-265 bp	1.0	79.2
trnG-GCC-trnM-CAU_p5	TTGCGGATATGGTCGAATGG	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	287-288 bp	1.0	79.0