Hotspot

trnG-UCC-trnR-UCU

LSC · 10429-10559 · 131 bp

Back to Kirengeshoma

Pi 0.0305

MarkerSeek score 70.10

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 4
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	211-214 bp	1.0	64.9
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	214-217 bp	1.0	64.6
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	217-220 bp	1.0	64.1
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	220-223 bp	1.0	63.9
trnG-UCC-trnR-UCU_p5	AGGAGCCGAATGAAACCAAA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	57.7	56.9	282-285 bp	1.0	62.4