Hotspot

psbI-trnS-GCU

LSC · 8692-8823 · 132 bp

Back to Kirengeshoma

Pi 0.0227

MarkerSeek score 65.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 132
variable_sites: 3
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.6	58.0	228 bp	1.0	77.8
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.6	58.0	229 bp	1.0	77.8
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.6	58.1	247 bp	1.0	76.9
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.6	58.4	226 bp	1.0	75.0
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.6	58.4	233 bp	1.0	74.8