primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	80	293	333-333	1.000000	77.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	80	293	347-347	1.000000	77.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	80	293	346-346	1.000000	77.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	80	293	348-348	1.000000	77.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	80	293	334-334	1.000000	77.0
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.403353	294	1355	1200-1200	1.000000	76.8
psbA_p2	psbA	2	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	294	1355	1270-1270	1.000000	76.4
psbA_p3	psbA	3	AAAGGAGCAATAACGCCCTC	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.627740	294	1355	1204-1204	1.000000	75.0
psbA_p4	psbA	4	AAAGGAGCAATAACGCCCTC	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.683935	294	1355	1274-1274	1.000000	74.6
psbA_p5	psbA	5	AGCAATAACGCCCTCTTGAT	ATTTGTGTGCTTGGGAGTCC	20	20	45.0	50.0	57.3	58.4	0.0	0.0	1.096816	294	1355	1199-1199	1.000000	71.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTCGCTTCATCAA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.1	58.0	0.0	7.6	0.084366	4147	4977	1005-1005	1.000000	79.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4147	4977	1018-1018	1.000000	79.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4147	4977	1017-1017	1.000000	79.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4147	4977	957-957	1.000000	79.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4147	4977	956-956	1.000000	79.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6131	7681	1633-1641	1.000000	79.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6131	7681	1616-1624	1.000000	79.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6131	7681	1634-1642	1.000000	78.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6131	7681	1631-1639	1.000000	77.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6131	7681	1630-1638	1.000000	77.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.401172	8692	8823	228-228	1.000000	77.8
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.402560	8692	8823	229-229	1.000000	77.8
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.506037	8692	8823	247-247	1.000000	76.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTAATCCTGGGCGTGAAGAA	GACTAAAGCGTCGGATTGCT	20	20	50.0	50.0	57.6	58.4	0.0	2.3	0.755013	8692	8823	226-226	1.000000	75.0
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	GTAATCCTGGGCGTGAAGAA	CTGAGTGGACTAAAGCGTCG	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.777729	8692	8823	233-233	1.000000	74.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10429	10559	211-214	1.000000	64.9
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10429	10559	214-217	1.000000	64.6
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10429	10559	217-220	1.000000	64.1
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10429	10559	220-223	1.000000	63.9
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AGGAGCCGAATGAAACCAAA	AGAAGACCTCTGTCCTATCCA	20	21	45.0	47.6	57.7	56.9	0.0	0.0	2.337969	10429	10559	282-285	1.000000	62.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31803	32556	885-895	1.000000	79.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31803	32556	864-874	1.000000	79.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31803	32556	861-871	1.000000	79.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31803	32556	847-857	1.000000	79.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31803	32556	826-836	1.000000	78.9
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	TTGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.147952	37407	37592	329-330	1.000000	79.5
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	TTGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.160570	37407	37592	293-294	1.000000	79.5
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195421	37407	37592	300-301	1.000000	79.2
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	37407	37592	264-265	1.000000	79.2
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	TTGCGGATATGGTCGAATGG	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.230154	37407	37592	287-288	1.000000	79.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45396	46247	939-941	1.000000	71.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45396	46247	960-962	1.000000	71.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	45396	46247	910-912	1.000000	70.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45396	46247	940-942	1.000000	69.1
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	45396	46247	937-939	1.000000	68.4
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	61901	62768	980-984	1.000000	79.3
ycf4-cemA_p2	ycf4-cemA	2	GGGGAAGTTGAGCAAAAAGC	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.231498	61901	62768	1007-1011	1.000000	78.8
ycf4-cemA_p3	ycf4-cemA	3	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	61901	62768	994-998	1.000000	78.6
ycf4-cemA_p4	ycf4-cemA	4	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	61901	62768	992-996	1.000000	78.3
ycf4-cemA_p5	ycf4-cemA	5	TTAACTCCCGGGGAAGTTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.3	58.1	29.2	0.0	0.344527	61901	62768	1016-1020	1.000000	77.9
petL-petG_p1	petL-petG	1	TGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	20	20	50.0	50.0	57.1	57.8	7.1	0.0	1.096307	67791	67961	304-304	1.000000	72.0
petL-petG_p2	petL-petG	2	TTGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	21	20	47.6	50.0	57.8	57.8	17.5	0.0	1.387067	67791	67961	305-305	1.000000	69.7
petL-petG_p3	petL-petG	3	GGTCTGAGCAAGATACGACT	CAACTGATCACCACGTCTGT	20	20	50.0	50.0	56.8	57.8	1.3	0.0	1.392364	67791	67961	303-303	1.000000	69.7
petL-petG_p4	petL-petG	4	TGGTCTGAGCAAGATACGAC	CCAACTGATCACCACGTCTG	20	20	50.0	55.0	57.1	58.6	7.1	0.0	1.508939	67791	67961	305-305	1.000000	68.7
petL-petG_p5	petL-petG	5	TGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	20	20	50.0	50.0	57.1	57.4	7.1	0.0	1.510076	67791	67961	310-310	1.000000	68.7
rpl20-rps12_p1	rpl20-rps12	1	TTTGTTCTACGTCTCCGAGC	ACTAGGGTGTATGTGCGACT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.494258	70727	71490	829-835	1.000000	76.9
rpl20-rps12_p2	rpl20-rps12	2	GTAAGTCGTGAATGAGCCCC	ACTAGGGTGTATGTGCGACT	20	20	55.0	50.0	58.3	58.2	0.0	0.0	0.498197	70727	71490	877-883	1.000000	76.8
rpl20-rps12_p3	rpl20-rps12	3	TGTTCTACGTCTCCGAGCTA	ACTAGGGTGTATGTGCGACT	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.542964	70727	71490	827-833	1.000000	76.5
rpl20-rps12_p4	rpl20-rps12	4	TAAGTCGTGAATGAGCCCCT	ACTAGGGTGTATGTGCGACT	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.582844	70727	71490	876-882	1.000000	76.2
rpl20-rps12_p5	rpl20-rps12	5	TTGTTCTACGTCTCCGAGCT	ACTAGGGTGTATGTGCGACT	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.619926	70727	71490	828-834	1.000000	75.9
rpl14-rpl16_p1	rpl14-rpl16	1	AGCATATCGGCGATTGCTAG	TGAAACGGGTGGAGTAACAG	20	20	50.0	50.0	58.0	57.5	35.6	0.0	0.538573	82800	82936	332-333	1.000000	76.7
rpl14-rpl16_p2	rpl14-rpl16	2	CATATCGGCGATTGCTAGCT	TGAAACGGGTGGAGTAACAG	20	20	50.0	50.0	58.0	57.5	28.9	0.0	0.538573	82800	82936	330-331	1.000000	76.7
rpl14-rpl16_p3	rpl14-rpl16	3	AGCATATCGGCGATTGCTAG	ACGGGTGGAGTAACAGAAAC	20	20	50.0	50.0	58.0	57.5	35.6	0.0	0.540324	82800	82936	328-329	1.000000	76.7
rpl14-rpl16_p4	rpl14-rpl16	4	CATATCGGCGATTGCTAGCT	ACGGGTGGAGTAACAGAAAC	20	20	50.0	50.0	58.0	57.5	28.9	0.0	0.540324	82800	82936	326-327	1.000000	76.7
rpl14-rpl16_p5	rpl14-rpl16	5	GAGCATATCGGCGATTGCTA	TGAAACGGGTGGAGTAACAG	20	20	50.0	50.0	58.0	57.5	36.0	0.0	0.540469	82800	82936	333-334	1.000000	76.6
ndhF-rpl32_p1	ndhF-rpl32	1	TGCCGTCGGAAAAAGAAGAA	GCTGCCCAATATCCCTTCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.415361	114106	115114	1188-1200	1.000000	77.3
ndhF-rpl32_p2	ndhF-rpl32	2	TTGCCGTCGGAAAAAGAAGA	GCTGCCCAATATCCCTTCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.415361	114106	115114	1189-1201	1.000000	77.3
ndhF-rpl32_p3	ndhF-rpl32	3	TGCCGTCGGAAAAAGAAGAA	CCTTTAGCGCTGCCCAATAT	20	20	45.0	50.0	58.0	58.4	17.5	22.9	0.434495	114106	115114	1196-1208	1.000000	77.1
ndhF-rpl32_p4	ndhF-rpl32	4	TTGCCGTCGGAAAAAGAAGA	CCTTTAGCGCTGCCCAATAT	20	20	45.0	50.0	58.0	58.4	17.5	22.9	0.434495	114106	115114	1197-1209	1.000000	77.1
ndhF-rpl32_p5	ndhF-rpl32	5	ACGAAAGGTATGATCCACGC	GCTGCCCAATATCCCTTCTT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.435725	114106	115114	1133-1145	1.000000	77.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.379488	115277	116199	1051-1051	1.000000	78.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGAAGGGATATTGGGCAGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.380859	115277	116199	1052-1052	1.000000	78.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ATATTGGGCAGCGCTAAAGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.4	58.0	11.0	0.0	0.398622	115277	116199	1043-1043	1.000000	78.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ATATTGGGCAGCGCTAAAGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.4	58.0	11.0	0.0	0.399993	115277	116199	1044-1044	1.000000	78.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGATATTGGGCAGCGCTAAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.4	58.0	11.0	0.0	0.403227	115277	116199	1045-1045	1.000000	78.1
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGATCCCCCGTTTTT	ATGGCAGTCGTTTGGGTATG	20	20	45.0	50.0	57.3	58.2	0.0	0.0	0.917951	125452	125845	518-518	1.000000	73.6
rps15-ycf1_p2	rps15-ycf1	2	TTCAACAGATCCCCCGTTTT	ATGGCAGTCGTTTGGGTATG	20	20	45.0	50.0	57.3	58.2	0.0	0.0	0.917951	125452	125845	519-519	1.000000	73.6
rps15-ycf1_p3	rps15-ycf1	3	TCAACAGATCCCCCGTTTTT	GGCAGTCGTTTGGGTATGTT	20	20	45.0	50.0	57.3	58.5	0.0	0.0	1.137821	125452	125845	516-516	1.000000	71.8
rps15-ycf1_p4	rps15-ycf1	4	TTCAACAGATCCCCCGTTTT	GGCAGTCGTTTGGGTATGTT	20	20	45.0	50.0	57.3	58.5	0.0	0.0	1.137821	125452	125845	517-517	1.000000	71.8
rps15-ycf1_p5	rps15-ycf1	5	ATTCAACAGATCCCCCGTTT	ATGGCAGTCGTTTGGGTATG	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.149110	125452	125845	520-520	1.000000	71.7
ycf1_p1	ycf1	1	GAATTCTGCTTCCGTGTTGC	TATTGAGGCATGGATCGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.493353	126937	127056	592-592	1.000000	77.8
ycf1_p2	ycf1	2	TACAGACAGACGAAGGGGTT	TATTGAGGCATGGATCGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.467826	126937	127056	838-838	1.000000	77.5
ycf1_p3	ycf1	3	TTACAGACAGACGAAGGGGT	TATTGAGGCATGGATCGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.467826	126937	127056	839-839	1.000000	77.5
ycf1_p4	ycf1	4	AGAAGGGGTCCAAGATCTCC	TATTGAGGCATGGATCGAGC	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.576034	126937	127056	492-492	1.000000	77.3
ycf1_p5	ycf1	5	GAAGGGGTCCAAGATCTCCT	TATTGAGGCATGGATCGAGC	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.576034	126937	127056	491-491	1.000000	77.3