Hotspot

ycf1

SSC · 127855-133587 · 5733 bp

Back to Kielmeyera

Pi 0.00401

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5733
variable_sites: 23
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCA	40.0	33.3	53.2	52.8	166 bp	1.0	42.4
ycf1_p2	TGATTTGCTTGGTATTACCC	AAGAAGTCAATTCATCCAATCA	40.0	31.8	53.2	53.7	167 bp	1.0	42.4
ycf1_p3	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCAA	40.0	31.8	53.2	53.7	166 bp	1.0	42.4
ycf1_p4	TGATTTGCTTGGTATTACCC	AAGAAGTCAATTCATCCAATCAA	40.0	30.4	53.2	54.5	167 bp	1.0	42.4
ycf1_p5	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCAAA	40.0	30.4	53.2	54.5	166 bp	1.0	42.4