Hotspot

psbL

LSC · 66959-67075 · 117 bp

Back to Kielmeyera

Pi 0.496

MarkerSeek score 72.60

Primer available yes

Label psbL

Feature Metadata

label_name: psbL
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 58
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL_p1	TCCTTCCAGTAGTATCGGCC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	492 bp	0.5	57.6
psbL_p2	TCCTTCCAGTAGTATCGGCC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	491 bp	0.5	57.6
psbL_p3	TCCTTCCAGTAGTATCGGCC	GAGAACCGACAAGGGATTCC	55.0	55.0	58.3	58.0	518 bp	0.5	57.4
psbL_p4	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	677 bp	0.5	57.3
psbL_p5	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	676 bp	0.5	57.3