Hotspot

psbJ

LSC · 66693-66815 · 123 bp

Back to Kielmeyera

Pi 0.515

MarkerSeek score 73.30

Primer available yes

Label psbJ

Feature Metadata

label_name: psbJ
feature_type: gene
region: LSC
length_bp: 123
variable_sites: 51
pi: 0.515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ_p1	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	677 bp	0.5	57.3
psbJ_p2	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	676 bp	0.5	57.3
psbJ_p3	TAAGAACTCAGTGGGACCCC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	687 bp	0.5	57.2
psbJ_p4	TAAGAACTCAGTGGGACCCC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	686 bp	0.5	57.2
psbJ_p5	GTGGGACCCCCTTTTCTCTA	GAGAACCGACAAGGGATTCC	55.0	55.0	58.3	58.0	703 bp	0.5	57.1