Hotspot

psbE

LSC · 67231-67482 · 252 bp

Back to Kielmeyera

Pi 0.527

MarkerSeek score 77.30

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: LSC
length_bp: 252
variable_sites: 125
pi: 0.527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	TCGTTCGGGTTTGATTGTGT	ATTGAAGTTGCGTTGCTGTG	45.0	45.0	58.0	57.9	603 bp	0.5	58.7
psbE_p2	GCATTGCGGATATAGACCCC	ATTGAAGTTGCGTTGCTGTG	55.0	45.0	58.3	57.9	537 bp	0.5	56.9
psbE_p3	TCCTTCCAGTAGTATCGGCC	ATTGAAGTTGCGTTGCTGTG	55.0	45.0	58.3	57.9	862 bp	0.5	56.6
psbE_p4	CGTTCGGGTTTGATTGTGTC	ATTGAAGTTGCGTTGCTGTG	50.0	45.0	57.7	57.9	602 bp	0.5	56.5
psbE_p5	TCGTTCGGGTTTGATTGTGT	TGATTGAAGTTGCGTTGCTG	45.0	45.0	58.0	57.6	605 bp	0.5	56.4