Hotspot

psbE-petL

LSC · 67483-68693 · 1211 bp

Back to Kielmeyera

Pi 0.24

MarkerSeek score 83.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1211
variable_sites: 270
pi: 0.24
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2014 bp	0.5	59.8
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2013 bp	0.5	59.8
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1992 bp	0.5	59.7
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1991 bp	0.5	59.7
psbE-petL_p5	GGAATCCCTTGTCGGTTCTC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1987 bp	0.5	59.7