Hotspot

psbL-psbF

LSC · 67076-67101 · 26 bp

Back to Kielmeyera

Pi 0.462

MarkerSeek score 72.50

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 12
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	AACTGGCCGTTTTGATCCTT	45.0	45.0	58.0	58.0	233 bp	0.5	59.6
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	ACTGGCCGTTTTGATCCTTT	45.0	45.0	58.0	58.0	232 bp	0.5	59.6
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	GAGAACCGACAAGGGATTCC	45.0	55.0	58.0	58.0	259 bp	0.5	59.4
psbL-psbF_p4	TCGTTCGGGTTTGATTGTGT	TGGTTATTCGTCAGCACTGG	45.0	50.0	58.0	57.9	328 bp	0.5	58.9
psbL-psbF_p5	TCGTTCGGGTTTGATTGTGT	GGTTATTCGTCAGCACTGGT	45.0	50.0	58.0	57.9	327 bp	0.5	58.9