Hotspot

psbJ-psbL

LSC · 66816-66958 · 143 bp

Back to Kielmeyera

Pi 0.496

MarkerSeek score 73.90

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 143
variable_sites: 70
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTTCCAGTAGTATCGGCC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	492 bp	0.5	57.6
psbJ-psbL_p2	TCCTTCCAGTAGTATCGGCC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	491 bp	0.5	57.6
psbJ-psbL_p3	TCCTTCCAGTAGTATCGGCC	GAGAACCGACAAGGGATTCC	55.0	55.0	58.3	58.0	518 bp	0.5	57.4
psbJ-psbL_p4	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.3	58.0	677 bp	0.5	57.3
psbJ-psbL_p5	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.3	58.0	676 bp	0.5	57.3