Hotspot

petA-psbJ

LSC · 65996-66692 · 697 bp

Back to Kielmeyera

Pi 0.352

MarkerSeek score 82.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 236
pi: 0.352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TATGATACCTCCAGGGCCAG	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1534 bp	0.5	59.7
petA-psbJ_p2	TATGATACCTCCAGGGCCAG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1533 bp	0.5	59.7
petA-psbJ_p3	GAGGGCGAATCCATCAAACT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1500 bp	0.5	59.6
petA-psbJ_p4	GAGGGCGAATCCATCAAACT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1499 bp	0.5	59.6
petA-psbJ_p5	TATGATACCTCCAGGGCCAG	GAGAACCGACAAGGGATTCC	55.0	55.0	58.0	58.0	1560 bp	0.5	59.6