Hotspot

psbZ-trnG-GCC

LSC · 38399-39256 · 858 bp

Back to Kielmeyera

Pi 0.0117

MarkerSeek score 66.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 10
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	1010-1044 bp	1.0	73.0
psbZ-trnG-GCC_p2	CTCCTGATGGTTGGTCAACT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	1009-1043 bp	1.0	73.0
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.9	57.8	1011-1045 bp	1.0	70.8
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	1012-1046 bp	1.0	70.6
psbZ-trnG-GCC_p5	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.4	57.8	1013-1047 bp	1.0	68.8