Hotspot

psbC-trnS-UGA

LSC · 37525-37748 · 224 bp

Back to Kielmeyera

Pi 0.0446

MarkerSeek score 78.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 10
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	328 bp	1.0	80.7
psbC-trnS-UGA_p2	AGCCTGTTCTTTCCATGACC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.8	58.1	330 bp	1.0	80.3
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	339 bp	1.0	80.0
psbC-trnS-UGA_p4	AGCCTGTTCTTTCCATGACC	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.8	58.1	341 bp	1.0	79.7
psbC-trnS-UGA_p5	GCAGCAGGATTCGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.7	58.1	367 bp	1.0	79.6