Hotspot

trnS-CGA-trnR-UCU

LSC · 10321-11011 · 691 bp

Back to Kielmeyera

Pi 0.0263
MarkerSeek score 74.80
Primer available yes
Label trnS-CGA-trnR-UCU

Feature Metadata

label_name
trnS-CGA-trnR-UCU
feature_type
igs
region
LSC
length_bp
691
variable_sites
18
pi
0.0263
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTTTGTCCTATCCA 50.0 42.9 58.0 55.7 772-784 bp 1.0 55.8
trnS-CGA-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTTTGTCCT 50.0 40.9 58.0 56.7 778-790 bp 1.0 55.6
trnS-CGA-trnR-UCU_p3 CCTAGCCTTCCAAGCTAACG AGAAGACCTTTGTCCTATCCA 55.0 42.9 58.1 55.7 775-787 bp 1.0 55.6
trnS-CGA-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTTTGTCCT 55.0 40.9 58.1 56.7 781-793 bp 1.0 55.3
trnS-CGA-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTTTGTCCTATCCA 55.0 42.9 57.7 55.7 776-788 bp 1.0 53.4