primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	trnS-CGA-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTTTGTCCTATCCA	20	21	50.0	42.9	58.0	55.7	0.0	0.0	3.343678	10321	11011	772-784	1.000000	55.8
trnS-CGA-trnR-UCU_p2	trnS-CGA-trnR-UCU	2	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTTTGTCCT	20	22	50.0	40.9	58.0	56.7	0.0	0.0	3.370207	10321	11011	778-790	1.000000	55.6
trnS-CGA-trnR-UCU_p3	trnS-CGA-trnR-UCU	3	CCTAGCCTTCCAAGCTAACG	AGAAGACCTTTGTCCTATCCA	20	21	55.0	42.9	58.1	55.7	0.0	0.0	3.372515	10321	11011	775-787	1.000000	55.6
trnS-CGA-trnR-UCU_p4	trnS-CGA-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTTTGTCCT	20	22	55.0	40.9	58.1	56.7	0.0	0.0	3.399044	10321	11011	781-793	1.000000	55.3
trnS-CGA-trnR-UCU_p5	trnS-CGA-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTTTGTCCTATCCA	20	21	55.0	42.9	57.7	55.7	0.0	0.0	3.647528	10321	11011	776-788	1.000000	53.4
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	37525	37748	328-328	1.000000	80.7
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	AGCCTGTTCTTTCCATGACC	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.265251	37525	37748	330-330	1.000000	80.3
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	37525	37748	339-339	1.000000	80.0
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	AGCCTGTTCTTTCCATGACC	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.335496	37525	37748	341-341	1.000000	79.7
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCAGCAGGATTCGAAAAAGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.327405	37525	37748	367-367	1.000000	79.6
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAAGACCGGAGCTATCAACC	GTTGACCAACCATCAGGAGA	20	20	55.0	50.0	58.1	57.1	9.5	1.6	0.917840	37839	38209	511-536	1.000000	73.0
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	CAAGACCGGAGCTATCAACC	AGTTGACCAACCATCAGGAG	20	20	55.0	50.0	58.1	57.1	9.5	9.1	0.925784	37839	38209	512-537	1.000000	72.9
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	CTATCAACCACTCGGCCATC	GTTGACCAACCATCAGGAGA	20	20	55.0	50.0	58.1	57.1	2.2	1.6	0.988085	37839	38209	500-525	1.000000	72.4
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CTATCAACCACTCGGCCATC	AGTTGACCAACCATCAGGAG	20	20	55.0	50.0	58.1	57.1	2.2	9.1	0.996029	37839	38209	501-526	1.000000	72.3
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	9.5	29.0	1.054924	37839	38209	440-465	1.000000	71.9
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	5.3	1.1	1.028998	38399	39256	1010-1044	1.000000	73.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTCCTGATGGTTGGTCAACT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	12.7	1.1	1.036941	38399	39256	1009-1043	1.000000	73.0
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	57.9	57.8	5.3	1.1	1.310157	38399	39256	1011-1045	1.000000	70.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	38399	39256	1012-1046	1.000000	70.6
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	58.4	57.8	0.0	1.1	1.549901	38399	39256	1013-1047	1.000000	68.8
petA-psbJ_p1	petA-psbJ	1	TATGATACCTCCAGGGCCAG	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.0	58.0	6.2	0.0	0.034651	65996	66692	1534-1534	0.500000	59.7
petA-psbJ_p2	petA-psbJ	2	TATGATACCTCCAGGGCCAG	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.0	58.0	6.2	0.0	0.034651	65996	66692	1533-1533	0.500000	59.7
petA-psbJ_p3	petA-psbJ	3	GAGGGCGAATCCATCAAACT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048746	65996	66692	1500-1500	0.500000	59.6
petA-psbJ_p4	petA-psbJ	4	GAGGGCGAATCCATCAAACT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048746	65996	66692	1499-1499	0.500000	59.6
petA-psbJ_p5	petA-psbJ	5	TATGATACCTCCAGGGCCAG	GAGAACCGACAAGGGATTCC	20	20	55.0	55.0	58.0	58.0	6.2	0.0	0.055616	65996	66692	1560-1560	0.500000	59.6
psbJ_p1	psbJ	1	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66693	66815	677-677	0.500000	57.3
psbJ_p2	psbJ	2	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66693	66815	676-676	0.500000	57.3
psbJ_p3	psbJ	3	TAAGAACTCAGTGGGACCCC	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.351677	66693	66815	687-687	0.500000	57.2
psbJ_p4	psbJ	4	TAAGAACTCAGTGGGACCCC	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.351677	66693	66815	686-686	0.500000	57.2
psbJ_p5	psbJ	5	GTGGGACCCCCTTTTCTCTA	GAGAACCGACAAGGGATTCC	20	20	55.0	55.0	58.3	58.0	8.4	0.0	0.360451	66693	66815	703-703	0.500000	57.1
psbJ-psbL_p1	psbJ-psbL	1	TCCTTCCAGTAGTATCGGCC	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	0.0	0.0	0.303669	66816	66958	492-492	0.500000	57.6
psbJ-psbL_p2	psbJ-psbL	2	TCCTTCCAGTAGTATCGGCC	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	0.0	0.0	0.303669	66816	66958	491-491	0.500000	57.6
psbJ-psbL_p3	psbJ-psbL	3	TCCTTCCAGTAGTATCGGCC	GAGAACCGACAAGGGATTCC	20	20	55.0	55.0	58.3	58.0	0.0	0.0	0.324633	66816	66958	518-518	0.500000	57.4
psbJ-psbL_p4	psbJ-psbL	4	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66816	66958	677-677	0.500000	57.3
psbJ-psbL_p5	psbJ-psbL	5	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66816	66958	676-676	0.500000	57.3
psbL_p1	psbL	1	TCCTTCCAGTAGTATCGGCC	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	0.0	0.0	0.303669	66959	67075	492-492	0.500000	57.6
psbL_p2	psbL	2	TCCTTCCAGTAGTATCGGCC	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	0.0	0.0	0.303669	66959	67075	491-491	0.500000	57.6
psbL_p3	psbL	3	TCCTTCCAGTAGTATCGGCC	GAGAACCGACAAGGGATTCC	20	20	55.0	55.0	58.3	58.0	0.0	0.0	0.324633	66959	67075	518-518	0.500000	57.4
psbL_p4	psbL	4	GTGGGACCCCCTTTTCTCTA	AACTGGCCGTTTTGATCCTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66959	67075	677-677	0.500000	57.3
psbL_p5	psbL	5	GTGGGACCCCCTTTTCTCTA	ACTGGCCGTTTTGATCCTTT	20	20	55.0	45.0	58.3	58.0	8.4	0.0	0.339486	66959	67075	676-676	0.500000	57.3
psbL-psbF_p1	psbL-psbF	1	TCGTTCGGGTTTGATTGTGT	AACTGGCCGTTTTGATCCTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67076	67101	233-233	0.500000	59.6
psbL-psbF_p2	psbL-psbF	2	TCGTTCGGGTTTGATTGTGT	ACTGGCCGTTTTGATCCTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67076	67101	232-232	0.500000	59.6
psbL-psbF_p3	psbL-psbF	3	TCGTTCGGGTTTGATTGTGT	GAGAACCGACAAGGGATTCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.069292	67076	67101	259-259	0.500000	59.4
psbL-psbF_p4	psbL-psbF	4	TCGTTCGGGTTTGATTGTGT	TGGTTATTCGTCAGCACTGG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.137009	67076	67101	328-328	0.500000	58.9
psbL-psbF_p5	psbL-psbF	5	TCGTTCGGGTTTGATTGTGT	GGTTATTCGTCAGCACTGGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.138465	67076	67101	327-327	0.500000	58.9
psbF_p1	psbF	1	TCGTTCGGGTTTGATTGTGT	AACTGGCCGTTTTGATCCTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67102	67221	233-233	0.500000	59.6
psbF_p2	psbF	2	TCGTTCGGGTTTGATTGTGT	ACTGGCCGTTTTGATCCTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67102	67221	232-232	0.500000	59.6
psbF_p3	psbF	3	TCGTTCGGGTTTGATTGTGT	GAGAACCGACAAGGGATTCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.069292	67102	67221	259-259	0.500000	59.4
psbF_p4	psbF	4	TCGTTCGGGTTTGATTGTGT	TGGTTATTCGTCAGCACTGG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.137009	67102	67221	328-328	0.500000	58.9
psbF_p5	psbF	5	TCGTTCGGGTTTGATTGTGT	GGTTATTCGTCAGCACTGGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.138465	67102	67221	327-327	0.500000	58.9
psbF-psbE_p1	psbF-psbE	1	TCGTTCGGGTTTGATTGTGT	AACTGGCCGTTTTGATCCTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67222	67230	233-233	0.500000	59.6
psbF-psbE_p2	psbF-psbE	2	TCGTTCGGGTTTGATTGTGT	ACTGGCCGTTTTGATCCTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.048327	67222	67230	232-232	0.500000	59.6
psbF-psbE_p3	psbF-psbE	3	TCGTTCGGGTTTGATTGTGT	GAGAACCGACAAGGGATTCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.069292	67222	67230	259-259	0.500000	59.4
psbF-psbE_p4	psbF-psbE	4	TCGTTCGGGTTTGATTGTGT	TGGTTATTCGTCAGCACTGG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.137009	67222	67230	328-328	0.500000	58.9
psbF-psbE_p5	psbF-psbE	5	TCGTTCGGGTTTGATTGTGT	GGTTATTCGTCAGCACTGGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.138465	67222	67230	327-327	0.500000	58.9
psbE_p1	psbE	1	TCGTTCGGGTTTGATTGTGT	ATTGAAGTTGCGTTGCTGTG	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.167714	67231	67482	603-603	0.500000	58.7
psbE_p2	psbE	2	GCATTGCGGATATAGACCCC	ATTGAAGTTGCGTTGCTGTG	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.384945	67231	67482	537-537	0.500000	56.9
psbE_p3	psbE	3	TCCTTCCAGTAGTATCGGCC	ATTGAAGTTGCGTTGCTGTG	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.423056	67231	67482	862-862	0.500000	56.6
psbE_p4	psbE	4	CGTTCGGGTTTGATTGTGTC	ATTGAAGTTGCGTTGCTGTG	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.433909	67231	67482	602-602	0.500000	56.5
psbE_p5	psbE	5	TCGTTCGGGTTTGATTGTGT	TGATTGAAGTTGCGTTGCTG	20	20	45.0	45.0	58.0	57.6	0.0	0.0	0.447968	67231	67482	605-605	0.500000	56.4
psbE-petL_p1	psbE-petL	1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	20	20	45.0	50.0	58.0	58.0	2.2	2.6	0.020651	67483	68693	2014-2014	0.500000	59.8
psbE-petL_p2	psbE-petL	2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	20	20	45.0	50.0	58.0	58.0	2.2	2.6	0.020651	67483	68693	2013-2013	0.500000	59.8
psbE-petL_p3	psbE-petL	3	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.041452	67483	68693	1992-1992	0.500000	59.7
psbE-petL_p4	psbE-petL	4	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.041452	67483	68693	1991-1991	0.500000	59.7
psbE-petL_p5	psbE-petL	5	GGAATCCCTTGTCGGTTCTC	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.0	58.0	0.0	2.6	0.041616	67483	68693	1987-1987	0.500000	59.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGATATTGGGCAGCCTTGAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	12.4	0.0	0.079608	117565	118358	898-928	1.000000	82.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGATATTGGGCAGCCTTGAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	12.4	0.0	0.080978	117565	118358	899-929	1.000000	82.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGATATTGGGCAGCCTTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	12.4	0.0	0.085518	117565	118358	900-930	1.000000	82.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGATATTGGGCAGCCTTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	12.4	0.0	0.086888	117565	118358	901-931	1.000000	82.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGATATTGGGCAGCCTTGAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	12.4	0.0	0.196055	117565	118358	908-938	1.000000	81.5
ycf1_p1	ycf1	1	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCA	20	21	40.0	33.3	53.2	52.8	10.4	0.0	10.977482	128904	129023	166-166	1.000000	42.4
ycf1_p2	ycf1	2	TGATTTGCTTGGTATTACCC	AAGAAGTCAATTCATCCAATCA	20	22	40.0	31.8	53.2	53.7	10.4	0.0	11.084096	128904	129023	167-167	1.000000	42.4
ycf1_p3	ycf1	3	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCAA	20	22	40.0	31.8	53.2	53.7	10.4	0.0	11.084096	128904	129023	166-166	1.000000	42.4
ycf1_p4	ycf1	4	TGATTTGCTTGGTATTACCC	AAGAAGTCAATTCATCCAATCAA	20	23	40.0	30.4	53.2	54.5	10.4	0.0	11.268813	128904	129023	167-167	1.000000	42.4
ycf1_p5	ycf1	5	TGATTTGCTTGGTATTACCC	AGAAGTCAATTCATCCAATCAAA	20	23	40.0	30.4	53.2	54.5	10.4	0.0	11.268813	128904	129023	166-166	1.000000	42.4