Hotspot

ycf3

LSC · 45043-47085 · 2043 bp

Pi 0.00346

MarkerSeek score 46.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2043
variable_sites: 14
pi: 0.00346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGCCCTATCCCTGTATCGTT	TTCGAGCCTGGGAGAAAAAG	50.0	50.0	57.9	57.8	2678-3000 bp	0.8	74.3
ycf3_p2	TGCTCAACGGACCTTTTGAA	ATACCCGCTATGCACAACTC	45.0	50.0	58.0	57.8	2876-2910 bp	0.8	70.2
ycf3_p3	TCTGCCCTATCCCTGTATCG	CTTCGAGCCTGGGAGAAAAA	55.0	50.0	58.1	57.8	2681-2995 bp	0.6	66.4
ycf3_p4	TCTGCCCTATCCCTGTATCG	TTCGAGCCTGGGAGAAAAAG	55.0	50.0	58.1	57.8	2680-2994 bp	0.6	66.4
ycf3_p5	TGCCCTATCCCTGTATCGTT	CTTCGAGCCTGGGAGAAAAA	50.0	50.0	57.9	57.8	2679-2993 bp	0.6	66.2