Hotspot

rps3

LSC · 86549-87208 · 660 bp

Pi 0.00212

MarkerSeek score 52.80

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 3
pi: 0.00212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCAACCCTATAGAATTGCGGA	GCAGACTATCGCAAACCAGA	42.9	50.0	56.5	58.0	857-858 bp	1.0	60.3
rps3_p2	TCAACCCTATAGAATTGCGGA	TGCAGACTATCGCAAACCAG	42.9	50.0	56.5	58.3	858-859 bp	1.0	58.2
rps3_p3	TCAACCCTATAGAATTGCGGAA	GCAGACTATCGCAAACCAGA	40.9	50.0	57.2	58.0	857-858 bp	1.0	58.1
rps3_p4	TTCAACCCTATAGAATTGCGGA	GCAGACTATCGCAAACCAGA	40.9	50.0	57.2	58.0	858-859 bp	1.0	58.1
rps3_p5	TCAACCCTATAGAATTGCGGA	ATGCAGACTATCGCAAACCA	42.9	45.0	56.5	57.6	859-860 bp	1.0	57.2