Hotspot

rpoC1

LSC · 22431-25236 · 2806 bp

Pi 0.00314

MarkerSeek score 53.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2806
variable_sites: 15
pi: 0.00314
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CTTGAACCGGAAAGACCCAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.0	58.0	2942-2995 bp	1.0	81.4
rpoC1_p2	TTGAACCGGAAAGACCCAAA	TTGCTCGTTCGAGAACTACG	45.0	50.0	57.6	58.0	2941-2994 bp	1.0	78.1
rpoC1_p3	CTTGAACCGGAAAGACCCAA	GCTCGTTCGAGAACTACGAT	50.0	50.0	58.0	57.5	2940-2993 bp	1.0	78.1
rpoC1_p4	ACCCAAATTCACACCACACC	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.6	58.0	2928-2981 bp	1.0	76.9
rpoC1_p5	ACCGGAAAGACCCAAATTCA	TTGCTCGTTCGAGAACTACG	45.0	50.0	57.3	58.0	2937-2990 bp	1.0	76.3