Hotspot

rpl22

LSC · 87193-87666 · 474 bp

Pi 0.0127

MarkerSeek score 46.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 474
variable_sites: 15
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.0	58.3	606-614 bp	1.0	79.1
rpl22_p2	CAAGTCGGAAACCAAGTGGA	AAATTGGGAGAATTTGCGCC	50.0	45.0	58.0	57.6	612-620 bp	1.0	78.6
rpl22_p3	ACCAAGTCGGAAACCAAGTG	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.3	58.3	608-616 bp	1.0	76.8
rpl22_p4	ACCAAGTCGGAAACCAAGTG	AAATTGGGAGAATTTGCGCC	50.0	45.0	58.3	57.6	614-622 bp	1.0	76.3
rpl22_p5	CAAGTCGGAAACCAAGTGGA	GCGCCCACTATAAATTTCCG	50.0	50.0	58.0	57.3	597-605 bp	1.0	76.2